To investigate the impact of anthocyanin on apoptosis of spinal cord ischemia-reperfusion injury rats and its
regulation on Toll-like receptor 4/cyclooxygenase 2/nuclear factor-kappa B signaling pathway. Sixty healthy
Sprague–Dawley rats were randomly separated into sham operation group, model group, anthocyanin low
(50 mg/kg), medium (100 mg/kg), high (150 mg/kg) concentration groups, anthocyanin (150 mg/kg)+TAK-242
(10 mg/kg) group, 10 per group, administered at 72 h before surgery. After the experiment, the behavioral
score after spinal cord injury was performed; blood was collected to separate serum, and the contents of
interleukin-6, interleukin-1 beta and tumor necrosis factor alpha were measured; the rats were sacrificed
to separate spinal cord tissue, and terminal deoxynucleotidyl transferase dUTP nick-end labeling staining
was used to detect cell apoptosis, the expression of B-cell lymphoma protein 2-associated X was measured
by immunohistochemistry, the expression of toll-like receptor 4, cyclooxygenase 2, p-nuclear factor-kappa
B and nuclear factor-kappa B proteins was measured by Western blot. Compared with the sham operation
group, the behavioral score and B-cell lymphoma protein 2-associated X expression in the model group
were obviously decreased (p<0.05); the apoptosis index, serum interleukin-6, interleukin-1 beta, tumor
necrosis factor alpha contents, B-cell lymphoma protein expression, toll-like receptor 4, cyclooxygenase 2
protein expression and p-nuclear factor-kappa B/nuclear factor-kappa B in spinal cord tissue were obviously
increased (p<0.05). Compared with the model group, the behavioral scores and B-cell lymphoma protein
2-associated X expression in the anthocyanin low, medium and high concentration groups were obviously
increased (p<0.05); the apoptosis index, serum interleukin-6, interleukin-1 beta, tumor necrosis factor alpha
contents, B-cell lymphoma protein 2 expression, toll-like receptor 4, cyclooxygenase 2 protein expression and
p-nuclear factor-kappa B/nuclear factor-kappa B in spinal cord tissue were gradually decreased (p<0.05).
Compared with the anthocyanin high concentration group, the behavioral score and B-cell lymphoma
protein 2-associated X expression in the anthocyanin+TAK-242 group were obviously increased (p<0.05);
the apoptosis index, serum interleukin-6, interleukin-1 beta, tumor necrosis factor alpha contents, B-cell
lymphoma protein 2 expression, toll-like receptor 4, cyclooxygenase 2 protein expression and p-nuclear
factor-kappa B/nuclear factor-kappa B in spinal cord tissue were obviously decreased (p<0.05). Anthocyanin
can ameliorate cell apoptosis in spinal cord ischemia-reperfusion injury rats by inhibiting the expression of
toll-like receptor 4/cyclooxygenase 2/nuclear factor-kappa B signaling pathway.